Targeted assessment of the metabolome in skeletal muscle and in serum of dairy cows supplemented with conjugated linoleic acid during early lactation

In the dairy cow, late gestation and early lactation are characterized by a complexity of metabolic processes required for homeorhetic adaptation to needs fetal growth milk production. Skeletal muscle plays an important role in this adaptation. The objective study was characterize metabolome skeletal (semitendinosus muscle) serum cows context physiological changes occurring test effects dietary supplementation (from d 1 onwards) with conjugated linoleic acids (sCLA; 100 g/d; supplying 7.6 g cis-9,trans-11 CLA trans-10,cis-12 per cow/d; n = 11) compared control fat-supplemented (CTR; 10). samples collected on 21 70 after calving conjunction their counterpart using targeted metabolomics approach (AbsoluteIDQ p180 kit; Biocrates Life Sciences AG, Innsbruck, Austria). Thereby 188 metabolites from 6 different compound classes (acylcarnitines, amino acids, biogenic amines, glycerophospholipids, sphingolipids, hexoses) were quantified both sample types. groups, dry matter intake increased calving. It lower sCLA than CTR 21, which resulted reduced calculated net energy metabolizable protein balances. On concentrations dopamine, Ala, hexoses higher CTR. changed mainly long-chain (>C24) diacyl phosphatidylcholine PC (PC-aa) acyl-alkyl (PC-ae), along lysophosphatidylcholine acyl (lysoPC-a) C26:1 that all Supplementation affected 22 including 10 (>C22) sphingomyelin (SM), hydroxysphingomyelin [SM(OH)], PC-aa, PC-ae 9 (>C16) lysoPC-a 3 related (spermine, citrulline, Asp). 70, C18:2 C26:0 cows. Regardless treatment, Ile, Leu, Phe, Lys, His, Met, Trp, hydroxybutyrylcarnitine (C4-OH) decreased, whereas those ornithine, Gln, trans-4-hydroxyproline (t4-OH-Pro) muscle. significantly time 28 (>C30) 7 SM SM(OH), C20:3 increased. conclusion, addition other metabolites, led glycerophospholipids sphingolipids might reflect phospholipid compositional observed seem be, at least part, due DMI these AA coincided steady MP balances, shift synthesis/degradation balance toward synthesis.